کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9256165 | 1211614 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bile-pancreatic juice exclusion increases p38MAPK activation and TNF-α production in ligation-induced acute pancreatitis in rats
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کلمات کلیدی
Donor Rat Modelp38 MAP kinase - p38 mAP kinaseAcinar cell - سلول AcinarCytokine - سیتوکینTNF-α - فاکتور نکروز توموری آلفاSignal transduction - هدایت سیگنالAcute pancreatitis - پانکراتیت حادPancreas - پانکراسStress-activated protein kinase - پروتئین کیناز فعال شده با استرسcholecystokinin - کولهسیستوکینین
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Acute pancreatitis is associated with stress kinase activation and cytokine production. We hypothesize that bilepancreatic juice exclusion activates p38MAPK and induces TNF-α production in ligation-induced acute pancreatitis. We compared rats with 1-3 h of duct ligation, duct ligation with duodenal bile-pancreatic juice replacement from a donor rat, and sham operation. Pancreatic homogenates were analyzed as follows: (a) Immunoblots using phospho-specific p38MAPK antibody showed increased p38MAPK activation after ligation that was inhibited by bile-pancreatic juice replacement. (b) Immune-complex kinase assay using ATF-2 as substrate showed increased p38MAPK activation after ligation that was subdued by bile-pancreatic juice replacement. (c) ELISA showed increased pancreatic TNF-α production after ligation that was significantly ameliorated by bile-pancreatic juice replacement. Conclusion: Bile-pancreatic juice exclusion from gut increases p38MAPK activation and TNF-α production in this experimental model. Our findings support our central hypothesis that bile-pancreatic juice exclusion exacerbates cell stress and acute inflammation in ligation-induced acute pancreatitis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pancreatology - Volume 5, Issue 1, 2005, Pages 20-26
Journal: Pancreatology - Volume 5, Issue 1, 2005, Pages 20-26
نویسندگان
Isaac Samuel, Smita Zaheer, Asgar Zaheer,