کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9279372 | 1593514 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Significance of avidity and immunoblot analysis for rubella IgM-positive serum samples in pregnant women
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
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چکیده انگلیسی
A total of 512 IgM-positive serum samples from 449 pregnant women referred by microbiologists and medical laboratories for additional testing and final interpretation were collected over a 3-year period. Employment of an IgM capture enzyme immunoassay (EIA) confirmed only 31% of the initial EIA-IgM-positive samples. In order to discriminate acute rubella virus infections, which are associated with increased risk of fetal infection and embryopathy, from persistent or non-specific IgM, IgG avidity index and the presence of IgG with specificity to rubella virus E2 glycoprotein was determined by Western immunoblot. In only six patients (1.3%), a primary infection with rubella virus was diagnosed on the basis of IgM positivity, low avidity IgG, absence of E2-specific IgG in immunoblot concordant with clinical findings as well as consistent changes in follow-up samples. The serological results were not compatible with rubella re-infection. The infection status in 14 patients (3.1%) remained inconclusive even when both avidity assay and immunoblot were used, while restriction to either test did not allow a conclusive interpretation in 11.6% of patients. The use of both assays is clearly better, and therefore, recommended in IgM-positive samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 130, Issues 1â2, December 2005, Pages 66-71
Journal: Journal of Virological Methods - Volume 130, Issues 1â2, December 2005, Pages 66-71
نویسندگان
J. Hofmann, U.G. Liebert,