کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9279385 1593514 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Real-time PCR assay for the detection of tanapox virus and yaba-like disease virus
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Real-time PCR assay for the detection of tanapox virus and yaba-like disease virus
چکیده انگلیسی
The yatapoxvirus genus contains three members: tanapox virus (TPV), yaba-like disease virus (YLDV) and yaba monkey tumor virus (YMTV), two of which (TPV and YLDV) may infect humans. However, only a very small number of patients have been diagnosed with TPV outside Africa. Given the increased international travel and the similarity of clinical signs during the early stages of a TPV/YLDV infection as compared to diseases caused by agents of potential biological warfare, such as smallpox, monkeypox, tularemia and anthrax, the rapid and reliable recognition of a TPV/YLDV infection is crucial. A real-time PCR assay using TaqMan® chemistry was developed in order to identify unambiguously TPV/YLDV. Primers and probe targeting a 101 bp region of the PstI L fragment of TPV, initial optimisations steps were carried out with YLDV DNA as template. Using probit regression analysis, the lower limit of detection was calculated to be ca. 8 copies per assay. A total of five TPV strains, one YDLV strain and scab-derived DNA from a patient with a TPV infection yielded specific amplification, whereas the DNA of YMTV was not amplified. Various viral and bacterial pathogens (n = 29) associated with rash-causing illnesses were not detected using this assay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 130, Issues 1–2, December 2005, Pages 149-153
نویسندگان
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