کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9279451 1223677 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A rapid and accurate assay for assessing the cytotoxicity of viral proteins
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
A rapid and accurate assay for assessing the cytotoxicity of viral proteins
چکیده انگلیسی
A fluorescence-based assay is presented for measuring the cytoxicity of viral proteins added exogenously to cells. The assay is based on the use of two fluorescent dyes, calcein-AM and ethidium homodimer (EtD-1) to specifically stain living and dead cells respectively and employs fluorescence activated cells sorting (FACS) to achieve a rapid and accurate measurement of the cytotoxic capacity of a potential viral toxin. The assay has been developed using the group B homologue (ADRV-NSP4) of the NSP4 enterotoxin encoded by Group A rotaviruses but should be applicable to assaying any viral protein exhibiting cytotoxic activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 127, Issue 2, August 2005, Pages 119-125
نویسندگان
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