کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9286850 | 1227377 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The N-terminal domain of the vaccinia virus E3L-protein is required for neurovirulence, but not induction of a protective immune response
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The N-terminal domain of the vaccinia virus E3L-protein is required for neurovirulence, but not induction of a protective immune response The N-terminal domain of the vaccinia virus E3L-protein is required for neurovirulence, but not induction of a protective immune response](/preview/png/9286850.png)
چکیده انگلیسی
Encephalitis is a rare, but serious complication from vaccination against smallpox using replication competent strains of vaccinia virus. In this report we describe mutants of vaccinia virus, containing N-terminal deletions of the vaccinia virus interferon resistance gene, E3L, that are attenuated for neuropathogenesis in a mouse model system. These recombinant viruses replicated to high titers in the nasal mucosa after intra-nasal infection of C57BL/6 mice but failed to spread to the lungs or brain. These viruses demonstrated reduced pathogenicity after intra-cranial infection as well, indicating a decrease in neurovirulence. Intra-nasal inoculation or inoculation by scarification with a low dose of recombinant virus containing a deletion of the entire N-terminal domain of E3L protected against challenge with a high dose of wild-type vaccinia virus, suggesting that this replication competent, but attenuated strain of vaccinia virus may have promise as an improved vaccine for protecting against smallpox, and as a vector for inducing mucosal immunity to heterologous pathogenic organisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 333, Issue 2, 15 March 2005, Pages 263-270
Journal: Virology - Volume 333, Issue 2, 15 March 2005, Pages 263-270
نویسندگان
Teresa Brandt, Michael C. Heck, Sangeetha Vijaysri, Garilyn M. Jentarra, Jason M. Cameron, Bertram L. Jacobs,