کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9287026 | 1227389 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A newly identified interaction between IVa2 and pVIII proteins during porcine adenovirus type 3 infection
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A newly identified interaction between IVa2 and pVIII proteins during porcine adenovirus type 3 infection A newly identified interaction between IVa2 and pVIII proteins during porcine adenovirus type 3 infection](/preview/png/9287026.png)
چکیده انگلیسی
The adenovirus IVa2 is an intermediate viral gene product that appears to perform multiple essential roles in viral infection. Using IVa2 as bait in the yeast two-hybrid system, we screened selected open reading frames (ORFs) of porcine adenovirus (PAdV)-3 for potential interaction with IVa2. Interestingly, pVIII showed specific interaction with IVa2. The yeast two-hybrid findings were validated by GST pull-down assays, in vitro binding studies employing cell-free coupled transcription-translation products and in vitro co-immunoprecipitations using protein-specific antibodies. Finally, we demonstrated that IVa2 specifically interacts with pVIII during PAdV-3 infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 336, Issue 1, 25 May 2005, Pages 60-69
Journal: Virology - Volume 336, Issue 1, 25 May 2005, Pages 60-69
نویسندگان
Mahavir Singh, Maya Shmulevitz, Suresh Kumar Tikoo,