کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9287282 | 1227405 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structure of avian orthoreovirus virion by electron cryomicroscopy and image reconstruction
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Structure of avian orthoreovirus virion by electron cryomicroscopy and image reconstruction Structure of avian orthoreovirus virion by electron cryomicroscopy and image reconstruction](/preview/png/9287282.png)
چکیده انگلیسی
Among members of the genus Orthoreovirus, family Reoviridae, a group of non-enveloped viruses with genomes comprising ten segments of double-stranded RNA, only the “non-fusogenic” mammalian orthoreoviruses (MRVs) have been studied to date by electron cryomicroscopy and three-dimensional image reconstruction. In addition to MRVs, this genus comprises other species that induce syncytium formation in cultured cells, a property shared with members of the related genus Aquareovirus. To augment studies of these “fusogenic” orthoreoviruses, we used electron cryomicroscopy and image reconstruction to analyze the virions of a fusogenic avian orthoreovirus (ARV). The structure of the ARV virion, determined from data at an effective resolution of 14.6 Ã
, showed strong similarities to that of MRVs. Of particular note, the ARV virion has its pentameric λ-class core turret protein in a closed conformation as in MRVs, not in a more open conformation as reported for aquareovirus. Similarly, the ARV virion contains 150 copies of its monomeric Ï-class core-nodule protein as in MRVs, not 120 copies as reported for aquareovirus. On the other hand, unlike that of MRVs, the ARV virion lacks “hub-and-spokes” complexes within the solvent channels at sites of local sixfold symmetry in the incomplete T = 13l outer capsid. In MRVs, these complexes are formed by C-terminal sequences in the trimeric μ-class outer-capsid protein, sequences that are genetically missing from the homologous protein of ARVs. The channel structures and C-terminal sequences of the homologous outer-capsid protein are also genetically missing from aquareoviruses. Overall, the results place ARVs between MRVs and aquareoviruses with respect to the highlighted features.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 343, Issue 1, 5 December 2005, Pages 25-35
Journal: Virology - Volume 343, Issue 1, 5 December 2005, Pages 25-35
نویسندگان
Xing Zhang, Jinghua Tang, Stephen B. Walker, David O'Hara, Max L. Nibert, Roy Duncan, Timothy S. Baker,