کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9288362 | 1227471 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The majority of the nucleotides in the top loop of the genomic 3â² terminal stem loop structure are cis-acting in a West Nile virus infectious clone
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The flavivirus genome RNA terminates with a conserved 3â² stem loop (SL) structure that was shown to be essential for virus replication. A stretch of conserved nts is located in the top loop (TL) of this structure. Mutation of the TL nts (5â² ACAGUGC 3â²) in a WNV infectious clone indicated that 3 of the 7 TL nts (5â² ACAGUGC 3â²) are critical for virus replication. Mutation of 3 of the other nts reduced the efficiency of virus replication. The four 5â² TL nts are conserved in both mosquito- and tick-borne flavivirus genomes, while the TL 3â² C is conserved in mosquito-borne viruses. The conservation of two or three G-C base pairs in the TL flanking sequences suggests that a stable stem is necessary for precise presentation of the TL sequence. The TL may participate in RNA as well as protein interactions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 331, Issue 2, 20 January 2005, Pages 238-246
Journal: Virology - Volume 331, Issue 2, 20 January 2005, Pages 238-246
نویسندگان
Salwa Elghonemy, William G. Davis, Margo A. Brinton,