کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9289475 1228316 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and enzymatic characterization of NS2B-NS3 protease of Alkhurma virus, a class-4 flavivirus
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Identification and enzymatic characterization of NS2B-NS3 protease of Alkhurma virus, a class-4 flavivirus
چکیده انگلیسی
We report here on the identification of the ALKV NS2B and NS3 domains and the expression and purification of a catalytically active viral protease as a hexahistidine recombinant protein. Its enzymatic properties were characterized in vitro using a para-nitroanilide substrate. This constitutes the first characterization of the proteinase from a class-4 flavivirus. Our results indicate that the association of NS3 with a short segment of NS2B is necessary and sufficient for protease activity. The developed system could help to identify or design inhibitors potentially active as antiviral drugs against ALKV and other pathogenic flaviviruses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 107, Issue 1, January 2005, Pages 57-62
نویسندگان
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