کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9296454 | 1233533 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Insulin alters nuclear factor-κB and peroxisome proliferator-activated receptor-γ protein expression induced by glycated bovine serum albumin in vascular smooth-muscle cells
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کلمات کلیدی
PGJ2VSMCRAGEFCSPAGEI-κBGoto-KakizakiAGE-BSAN-2-hydroxyethylpiperazine-N-2-ethanesulfonic acidHDLHEPESglycated Bovine Serum AlbuminSDSDMEMPBSNF-κBPPAR-γBSA - BSAhigh-density lipoprotein - HDL یا لیپوپروتئین با دانسیته بالا یا چگالی بالاDulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoROS - ROSbovine serum albumin - آلبومین سرم گاوpolyacrylamide-gel electrophoresis - الکتروفورز پلی آکریل آمید ژلsodium dodecyl sulfate - سدیم دودسیل سولفاتVascular smooth muscle cell - سلول عضله صاف عروقیAge - سنLow-density lipoprotein - لیپوپروتئین کم چگالی یا الدیال LDL - لیپوپروتئین کم چگالی(کلسترول بد)phosphate-buffered saline solution - محلول نمک فسفات بافرinhibitor κB - مهار کننده κBNitric oxide - نیتریک اکسیدadvanced glycation end product - پیشرفته محصول نهایی گلیساسیونReactive oxygen species - گونههای فعال اکسیژنreceptor for AGE - گیرنده برای AGEPeroxisome proliferator-activated receptor-γ - گیرنده پروتئینی فعال پروکسیوم - γ
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In both type 2 diabetes and insulin-resistance syndromes, hyperglycemia and advanced glycation end products (AGEs) activate the transcription factor nuclear factor-κB (NF-κB) through a mechanism that partly involves the generation of reactive oxygen species (ROS). The contribution of hyperinsulinemia in this sequence has not been completely elucidated. In this work we investigated the actions of insulin and PPAR-γ on the stimulation by AGEs of NF-κB protein expression in cultured aortic vascular smooth-muscle cells (VSMCs) from non-insulin-dependent diabetic rats and nondiabetic rats. The expression of proteins was evaluated with the use of Western immunoblotting. Incubations (24 hours) of VSMCs with 10 to 100 μg/mL glycated bovine serum albumin (AGE- BSA) increased NF-κB protein expression in both models. PPAR-γ protein expression was only enhanced at concentrations of 500 to 1000 μg/mL (AGE-BSA). In the presence of insulin (10-100 nmol/L), the stimulation of NF-κB protein expression by AGE-BSA (100 μg/mL) was decreased, whereas the expression of PPAR-γ protein was enhanced. 15-Deoxy-prostaglandin J2, a direct activator of PPAR-γ, decreased AGE-BSA-stimulated NF-κB expression. These findings suggest that insulin decreases the incidence of alterations in VSMCs induced by AGEs through the reduction of NF-κB and an increase in PPAR-γ protein expression (as far as the model could be extrapolated to in vivo situations). These data may help justify current therapeutic approaches involving the use of insulin and PPAR-γ agonists.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Laboratory and Clinical Medicine - Volume 145, Issue 3, March 2005, Pages 144-150
Journal: Journal of Laboratory and Clinical Medicine - Volume 145, Issue 3, March 2005, Pages 144-150
نویسندگان
Cristina de Oliveira, Claude Colette, Louis Monnier, Bernard Descomps, Nuria Pares-Herbute,