کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9367313 | 1272023 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Erythrocyte membrane fluidity and haemoglobin haemoporphyrin conformation: features revealed in patients with heart failure
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
آسیبشناسی و فناوری پزشکی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study examined the possible involvement of abnormal erythrocyte oxygen (O2) transport in the pathogenesis of heart failure. Haemoglobin (Hb) haemoporphyrin conformation was assessed by Raman spectroscopy (RS) of blood samples, whereas membrane fluidity was estimated at depths of 0.6-0.8 and 2.2Â nm by electron-paramagnetic resonance spectroscopy of erythrocytes loaded with spin-labeled 5-doxylstearic acid and 16-doxylstearic acid, respectively. The fluidity of erythrocyte membranes from patients with heart failure was decreased in the area near the membrane surface and remained unchanged in the deeper hydrophobic membrane regions. The same differences were also detected in healthy controls subjected to chronic high-altitude hypoxia. RS demonstrated that in heart failure the total content of Hb-ligand complexes and the relative content of Hb-nitric oxide (NO) complexes with cleaved Fe2+-globin bond was decreased, whereas content of Hb-NO complexes with preserved Fe2+-globin bond was increased. We propose that this phenomenon contributes to the reduced O2 tissue supply seen in patients with heart failure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pathophysiology - Volume 11, Issue 4, May 2005, Pages 209-213
Journal: Pathophysiology - Volume 11, Issue 4, May 2005, Pages 209-213
نویسندگان
O.V. Rodnenkov, O.G. Luneva, N.A. Ulyanova, G.V. Maksimov, A.B. Rubin, S.N. Orlov, E.I. Chazov,