کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9407 628 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biopolymer mediated trehalose uptake for enhanced erythrocyte cryosurvival
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Biopolymer mediated trehalose uptake for enhanced erythrocyte cryosurvival
چکیده انگلیسی

A biopolymer has been shown to facilitate efficient delivery of trehalose, a bioprotectant normally impermeable to the phospholipid bilayer, into ovine erythrocytes. Cellular uptake of trehalose was found to be dependent on polymer pendant amino acid type and degree of grafting, polymer concentration, pH, external trehalose concentration, incubation temperature and time. Optimization of these parameters yielded an intracellular trehalose concentration of 123 ± 16 mm and concomitant improvement of erythrocyte cryosurvival of up to 20.4 ± 5.6%. Intracellular trehalose was shown to impart cellular osmoprotection up to an external osmolarity of 230 mOsm and increased osmotic sensitivity above this threshold. Biopolymer mediated membrane permeability was shown to be rapidly and completely reversible via washing with phosphate buffered saline.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 31, Issue 23, August 2010, Pages 6096–6103
نویسندگان
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