کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9410524 | 1613315 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adenoviral interneuronal transportation after retrograde gene transfer in mice
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کلمات کلیدی
GFAPeGFPRecombinant adenovirus - آدنویروس مجددMotor systems and sensorimotor integration - سیستم های موتور و ادغام سنسور حرکتیSpinal cord - طناب نخاعیSpinal cord and brainstem - نخاع و مغز استخوانMotor neuron - نورون حرکتیGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالenhanced green fluorescence protein - پروتئین فلورسانس سبز افزایش یافته استChAT - چتGene therapy - ژن درمانیcholine acetyltransferase - کولین استیل ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Although retrograde gene transfer from infected muscles to neurons by viral vectors has been known for years, it is still unknown whether interneuronal gene transportation of viral vectors occurs after retrograde gene transfer. To determine this, we injected adenoviral vectors carrying eGFP gene with or without a neural tracer into the right gastrocnemius muscles of mice. After 7 days, some spinal motor neurons were detected with green fluorescence but without the signal of neural tracer. In addition, nerves with green fluorescence could be noted in the right lumbosacral paraspinal muscles of viral-injected mice. The green fluorescence in the right lumbosacral paraspinal muscles might have resulted from retrograde gene transportation from the viral-injected gastrocnemius muscles to the spinal neurons, followed by interneuronal transfer and anterograde expression of eGFP in the axons belonging to neurons innervating the paraspinal muscles. This phenomenon of interneuronal transportation raises the possibility that we could treat motoneuron diseases by injection of viral vectors containing therapeutic genes into a few muscles resulting in widespread beneficial effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Brain Research - Volume 142, Issue 2, 14 December 2005, Pages 151-155
Journal: Molecular Brain Research - Volume 142, Issue 2, 14 December 2005, Pages 151-155
نویسندگان
Li-Kai Tsai, Ming-Shiun Tsai, Song-Kun Shyue, Wuh-Liang Hwu, Hung Li,