کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9410605 | 1291268 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunocytochemical localization of a neuron-specific diacylglycerol kinase β and γ in the developing rat brain
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کلمات کلیدی
Immunocytochemistry - ایمونوسیتوشیمیdiacylglycerol kinase - دیسیل گلیسرول کینازDevelopment - رشدStaining, tracing and imaging techniques - رنگ آمیزی، ردیابی و تکنیک های تصویربرداریSubtype - زیرمجموعهCellular and molecular biology - زیست شناسی سلولی و مولکولیBrain - مغزProtein kinase C - پروتئین کیناز سی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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چکیده انگلیسی
Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA) and is, therefore, a potential terminator of DG signaling. DG and PA are important intracellular second messengers. DG directly binds protein kinase C (PKC) then activates this multifunctional enzyme. Ca2+-dependent and brain-specific DGKs, α, β, and γ, are suggested to play pivotal roles in the central nervous system. To elucidate the DGK function in neuronal development, we studied the developmental changes of DGKα, β, and γ in the postnatal rat brain. By immunoblot analysis, DGKα and γ subtypes were present at birth and then gradually increased, while DGKβ was not present at birth or postnatal day 3, then increased rapidly from day 14 to reach maximum at day 28. Immunohistochemically, DGKβ and γ were distributed in different brain regions. In most brain regions, DGKγ showed sustained expression throughout the postnatal developmental periods. Interestingly, a temporal expression of DGKγ was observed in the medial geniculate nucleus during day 3 to 14, and a delay of DGKγ expression was seen in Purkinje cells, which was coincident with dendritic growth of Purkinje cells. In the hippocampal pyramidal cell, both DGKβ and γ were abundant but subcellular localization was different. DGKγ localized in the cytosol while DGKβ localized along the membrane structure. These findings suggest that each DGK subtype has a spatio-temporally different function in the developmental neurons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Brain Research - Volume 139, Issue 2, 3 October 2005, Pages 288-299
Journal: Molecular Brain Research - Volume 139, Issue 2, 3 October 2005, Pages 288-299
نویسندگان
Naoko Adachi, Miho Oyasu, Taizo Taniguchi, Yasuto Yamaguchi, Rika Takenaka, Yasuhito Shirai, Naoaki Saito,