کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9410681 | 1613319 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
p55 protein is a member of PSD scaffold proteins in the rat brain and interacts with various PSD proteins
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کلمات کلیدی
DemMPPPSDORFGSTRT-PCRSPMCaMKIIp55Ca2+/calmodulin-dependent protein kinase II - Ca2 + / calmodulin وابسته پروتئین کیناز IIMAGUK - آنهاpostsynaptic density - تراکم PostinapticProtein–protein interaction - تعامل پروتئین-پروتئینScaffold - داربَستExcitable membranes and synaptic transmission - غشاء جذاب و انتقال سیناپسیSynaptic plasma membrane - غشای پلاسمای سیناپتیکopen reading frame - قاب خواندن بازPostsynaptic mechanisms - مکانیزم های پستSynapse - همایه یا سیناپسpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازglutathione S-transferase - گلوتاتیون S-ترانسفرازGuanylate kinase - گینیلات کیناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
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چکیده انگلیسی
p55 is a membrane-associated guanylate kinase (MAGuK) family member that consists of a single PDZ followed by SH3, HOOK and guanylate kinase (GuK or GK) domains. We investigated rat p55 (r-p55) in the brain. r-p55 mRNA was expressed widely in various tissues and in various regions of the brain. r-p55 protein was also expressed widely in various rat tissues, including brain and erythrocytes. The protein was enriched in the synaptic plasma membrane and postsynaptic density (PSD) fractions of the forebrain. An immunocytochemical study using cultured cortical neurons suggested postsynaptic localization of r-p55 protein. Pull-down assay showed that r-p55 protein interacted with r-p55 itself and various PSD proteins, such as PSD-95, SAP97, GKAP, CASK, GRIP, neuroligin, cadherin, tubulin, actin, α-internexin, neurofilament-L and Ca2+/calmodulin-dependent protein kinase II, through its PDZ, SH3, HOOK or GK domains. The interaction with PSD-95 was found to occur between the PDZ domains of PSD-95 and the HOOK and GK domains of r-p55 protein. These findings, together with the presence of r-p55 puncta in a period of early synaptogenesis, suggest that r-p55 protein functions as one of postsynaptic scaffold component in an early stage of synaptogenesis in the brain. r-p55 protein may form a basic structure, which interlinks diverse functional molecules of the PSD necessary for postsynaptic signaling and synaptic adhesion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Brain Research - Volume 135, Issues 1â2, 27 April 2005, Pages 204-216
Journal: Molecular Brain Research - Volume 135, Issues 1â2, 27 April 2005, Pages 204-216
نویسندگان
Zhang Jing-Ping, Qing-Bao Tian, Hiroyuki Sakagami, Hisatake Kondo, Shogo Endo, Tatsuo Suzuki,