کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9425618 | 1295883 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pigment epithelium-derived factor induces pro-survival genes through cyclic AMP-responsive element binding protein and nuclear factor kappa B activation in rat cultured cerebellar granule cells: Implication for its neuroprotective effect
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کلمات کلیدی
IAPSEMSAcyclic AMP-response elementm.o.icyclic AMP-responsive element binding proteinN-acetyl-leu-leu-norleucinalALLNPEDFFLIPCGCsTBSTPI3-KCREBRT-PCRNF-κBCREERKMTSBSA - BSAMAPK - MAPKMn-SOD - MN-SODSERPIN - SERPIAelectrophoresis mobility shift assay - آزمایش تحرک تحرک الکتروفورزbovine serum albumin - آلبومین سرم گاوNeuronal apoptosis - آپوپتوز عصبیSerine protease inhibitor - بازدارنده Serine پروتئازDIV - دیوdays in vitro - روز in vitrocerebellar granule cells - سلول های گرانول مخچهmanganese superoxide dismutase - سوپر اکسید دیسموتاز منگنزPigment epithelium-derived factor - عامل مشتق شده از اپیتلیوم رنگدانهTranscription factors - عوامل رونویسیnuclear factor kappa B - فاکتور هسته ای کاپا BPhosphatidylinositol 3-kinase - فسفاتیدیلینواستیل 3-کینازSignal transduction pathway - مسیر انتقال سیگنالreverse transcriptase polymerase chain reaction - واکنش زنجیره ای پلی مراز ترانس کریتاز معکوسmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenmultiplicity of infection - چندین عفونتextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Pigment epithelium-derived factor (PEDF) protects immature cerebellar granule cell neurons (CGCs) against apoptosis induced by K+ and serum deprivation. However, the precise mechanism of this protection remains unknown. We recently reported that the transcription factor nuclear factor kappa B (NF-κB) is activated in PEDF-treated CGCs. Although it is well known that NF-κB blocks apoptotic cell death through the induction of pro-survival factors, the effects of PEDF on the expression of these factors are not fully understood. In this study, we employed the use of reverse transcriptase-polymerase chain reaction to analyze the gene expression of certain pro-survival genes and found that genes such as c-IAP1, c-IAP2, FLIPs, A1/Bfl-1 and Mn-SOD were induced in PEDF-treated neurons. On the other hand, no induction was observed of the pro-apoptotic Bcl-2 family members Bax and Bid at any time from 3 to 24 h following PEDF addition. Furthermore, phosphorylation of cyclic AMP-responsive element binding protein (CREB) and increment of nuclear cyclic AMP-response element (CRE)-like DNA binding were observed in PEDF-treated CGCs. The anti-apoptotic effect of PEDF was blocked by overexpression of dominant negative CREB or a mutated form of IκBα. These results suggested that induction of both CRE- and NF-κB-dependent genes is required for the observed neuroprotective effects of PEDF on CGCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 133, Issue 3, 2005, Pages 691-700
Journal: Neuroscience - Volume 133, Issue 3, 2005, Pages 691-700
نویسندگان
T. Yabe, K. Kanemitsu, T. Sanagi, J.P. Schwartz, H. Yamada,