کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9426719 | 1295932 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sargachromenol, a novel nerve growth factor-potentiating substance isolated from Sargassum macrocarpum, promotes neurite outgrowth and survival via distinct signaling pathways in PC12D cells
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کلمات کلیدی
PBSED50NGFpKaERKHEPESFBSJnkPI3KSDSDMEMc-Jun N-terminal protein kinase - C-Jun N-terminal protein kinaseN-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid] - N- [2-hydroxyethyl] piperazine-N '- [2-ethanesulfonic acid]EDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدsodium dodecyl sulfate - سدیم دودسیل سولفاتhorse serum - سرب اسبfetal bovine serum - سرم جنین گاوnerve growth factor - فاکتور رشد عصبphosphatidylinositol-3 kinase - فسفاتیدیلینوزیتول 3 کینازMethanol - متانولmedian effective dose - متوسط دوز موثرPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoMeOH - مونmap - نقشهmitogen-activated protein - پروتئین فعال mitogenprotein kinase A - پروتئین کیناز Amitogen-activated protein kinase - پروتئین کیناز فعال با mitogenextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
We previously found that the methanol extract of a marine brown alga, Sargassum macrocarpum showed marked nerve growth factor (NGF)-dependent neurite outgrowth promoting activity to PC12D cells. The active substance purified was elucidated to be sargachromenol. The median effective dose (ED50) was 9 μM against PC12D cells in the presence of 10 ng/ml NGF, although it showed no neurotrophic effect on its own. Pretreatment of cells with protein kinase A (PKA) inhibitor or U0126 substantially suppressed the sargachromenol-enhanced neurite outgrowth from PC12D cells, suggesting that the activation of cyclic AMP-mediated protein kinase and mitogen-activated protein (MAP) kinase 1/2 was apparently required for the action of sargachromenol. On the other hand, sargachromenol significantly promoted the survival of neuronal PC12D cells at 0-50 ng/ml NGF in serum-free medium. Neither PKA inhibitor nor U0126 could inhibit the survival supporting effect of sargachromenol, whereas wortmannin significantly blocked the sargachromenol-induced survival supporting effect on neuronal PC12D cells, suggesting that sargachromenol rescued neuronal PC12D cells by activating phosphatidylinositol-3 kinase. These results demonstrate that sargachromenol promotes neuronal differentiation of PC12D cells and supports the survival of neuronal PC12D cells via two distinct signaling pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 132, Issue 3, 2005, Pages 633-643
Journal: Neuroscience - Volume 132, Issue 3, 2005, Pages 633-643
نویسندگان
C.K. Tsang, A. Ina, T. Goto, Y. Kamei,