کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9429751 1297058 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular mechanisms responsible for microglia-derived protection of Sprague-Dawley rat brain cells during in vitro ischemia
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Molecular mechanisms responsible for microglia-derived protection of Sprague-Dawley rat brain cells during in vitro ischemia
چکیده انگلیسی
Microglia-derived protection of brain cells (microglia, astrocytes, and neurons) during in vitro ischemic stress (deprivation of glucose, oxygen, and serum) was determined. Trypan blue exclusion assay, immunoblocking assay, Western blot analysis, and ELISA assay were used to determine the molecular mechanisms responsible for the microglia-derived protection. Results demonstrated that supernatants from the ischemic microglia protected all three cell-types from ischemia-induced damage by releasing the transforming growth factor-β1 (TGF-β1) and glial cell line-derived neurotrophic factor (GDNF). The protection of microglia was TGF-β1 related, whereas astrocytes protection was GDNF-dependent. The protection of neurons was TGF-β1 and GDNF independent, and the molecular nature responsible for their protection remains to be determined. These results indicate contribution from the surrounding cells and the types of receptors expressed on different brain cells probably also play an important role in determining their fate against ischemia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 373, Issue 2, 10 January 2005, Pages 159-164
نویسندگان
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