کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9437622 | 1615784 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of bacteria with β-galactosidase activity in faeces from lactase non-persistent subjects
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بوم شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Previous studies suggest that, besides the maldigestion of lactose in the small intestine, the colonic processing of lactose might play a role in lactose intolerance. β-Galactosidase is the bacterial enzyme which catalyzes the first step of lactose fermentation in the colon. We propose a practical method to differentiate and identify bacteria with β-galactosidase activity in faeces which combines a colony-lift filter assay with X-gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) as substrate for differentiation and the fluorescent in situ hybridization technique for identification. The method was applied to faeces from lactase non-persistent subjects. After 28 subjects had undergone one glucose and two lactose challenges, consistent intolerant (n = 5) and tolerant (n = 7) groups were defined according to their symptom scores. Of the 28 faecal samples, 80.6% (mean, SD: 12.1, range: 47.8-100%) of the total cultured bacteria were found to possess β-galactosidase activity, which indicates that the bacterial β-galactosidase is abundant in the colon. The tolerant and intolerant groups did not differ in the percentage or composition of the bacteria with β-galactosidase activity or β-galactosidase activity in faeces. Results suggest that the percentage or composition of the bacteria with β-galactosidase activity in faeces do not play a role in lactose intolerance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Ecology - Volume 54, Issue 3, November 2005, Pages 463-469
Journal: FEMS Microbiology Ecology - Volume 54, Issue 3, November 2005, Pages 463-469
نویسندگان
Tao He, Marion G. Priebe, Roel J. Vonk, Gjalt W. Welling,