کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9437673 | 1615785 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of cation-exchange membranes for characterisation and imaging ammonia-oxidising bacteria in soils
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بوم شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Application of cation-exchange membranes for characterisation and imaging ammonia-oxidising bacteria in soils Application of cation-exchange membranes for characterisation and imaging ammonia-oxidising bacteria in soils](/preview/png/9437673.png)
چکیده انگلیسی
A new approach, in which ammonia-oxidizing bacteria (AOB) are entrapped from soil onto cation-exchange membranes, was applied to identify terrestrial AOB by fluorescence in situ hybridization (FISH). An experimental hot spot of ammonia oxidation was developed by establishing a gradient of ammonium substrate (200 to <20Â mg NH4+-Nl-1) diffused through the cation-exchange membranes incubated in soil for 6 months. By this approach we were able to characterise and image indigenous AOB populations growing in heavily oil-polluted soil using FISH and sequence analysis of PCR-amplified 16S rRNA genes, respectively. The FISH results revealed that Nitrosospira-like AOB were dominant on the ammonium-enriched membranes incubated in the soil. Fourteen unique Nitrosospira-like 16S rRNA gene sequences belonging to clusters 2 and 3 were recovered from the soil-incubated membranes and from the soil, suggesting the importance of Nitrosospira-like AOB in the oil-polluted landfarming soil.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Ecology - Volume 53, Issue 3, 1 August 2005, Pages 463-472
Journal: FEMS Microbiology Ecology - Volume 53, Issue 3, 1 August 2005, Pages 463-472
نویسندگان
Jukka Kurola, Christoph Wittmann, Mirja Salkinoja-Salonen, Tuula Aarnio, Martin Romantschuk,