کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9440050 | 1300535 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of the cytolethal distending toxin locus cdtB among diarrheagenic Escherichia coli isolates from humans in Iran
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Detection of the cytolethal distending toxin locus cdtB among diarrheagenic Escherichia coli isolates from humans in Iran Detection of the cytolethal distending toxin locus cdtB among diarrheagenic Escherichia coli isolates from humans in Iran](/preview/png/9440050.png)
چکیده انگلیسی
Cytolethal distending toxin (CDT) represents an emerging family of newly described bacterial products that are produced by a number of pathogens. Genes encoding this toxin were identified as a cluster of three adjacent genes, cdt-A, cdt-B and cdt-C. Five cdt genetic variants, designated cdt-I, cdt-II, cdt-III, cdt-IV and cdt-V, have been identified so far. To determine the presence of cdt genes among Escherichia coli isolates, a PCR assay was employed. Using multiplex primers designed for detection of E. coli cdt genes, PCR analysis indicated the presence of cdt genes in 37.5% of these isolates. While specific primers were located in the cdt-B locus for detection of cdt-I, cdt-II, cdt-III and cdt-IV, in multiplex PCR positive isolates indicated that a cdt-I-like gene was present in 45.3% of these isolates. However, in 52% of the isolates, the cdt-III-like gene could be detected. It should be mentioned that in 30.8% of these isolates, cdt-II and -III were detected simultaneously. In 2.6% of the isolates, the cdt-IV gene was present. The cnf-1 gene was detected in 29.4% of strains carrying the cdt-I gene; however, the cnf-2 gene was detected only in 23.1% of the cdt-III-like strains. Furthermore, the data obtained by PCR analysis indicated the presence of cdt-like genes among our E. coli isolates, although in the CHO cell assay, all isolates showed a cytopathic effect characteristic of CDT.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 156, Issue 2, March 2005, Pages 137-144
Journal: Research in Microbiology - Volume 156, Issue 2, March 2005, Pages 137-144
نویسندگان
Saeid Bouzari, Mana Oloomi, Eric Oswald,