کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9442961 | 1302612 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Entamoeba histolytica: Analysis of the trophozoite proteome by two-dimensional polyacrylamide gel electrophoresis
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کلمات کلیدی
E64DTTCAPSTCAPMSFIEF2DE - 2de3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate - 3 - [(3-کلامیدوپروپیل) دی متیل آمونیو] -1-پروپان سولفوناتEntamoeba histolytica - Entamoeba هیستولیتیکاIPG - GPAimmobilized pH gradient - gradient pH immobilizedtrichloroacetic acid - اسید ترشکلراکتیکtwo-dimensional gel electrophoresis - الکتروفورز ژل دو بعدیisoelectric focusing - تمرکز ذره ای الکتریکیdithiothreitol - دیتیوتریتولMass spectrometry - طیف سنجی جرمیphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدMALDI-TOF MS - مالدی توف MSProteomics - پروتئومیکسCHAPS - چاپس
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Entamoeba histolytica: Analysis of the trophozoite proteome by two-dimensional polyacrylamide gel electrophoresis Entamoeba histolytica: Analysis of the trophozoite proteome by two-dimensional polyacrylamide gel electrophoresis](/preview/png/9442961.png)
چکیده انگلیسی
The Entamoeba histolytica genome project carried out at TIGR and the Sanger Institute has produced a near-complete set of deduced open reading frame sequences. These data provide strong support for the identification of signals from proteomic analyses such as two-dimensional electrophoresis by protein sequencing and/or mass spectrometric methods. To carry out an initial investigation of the E. histolytica proteome, appropriate sample preparation and silver staining protocols were adapted. After preparation of protein extracts from E. histolytica HM-1:IMSS trophozoites, solubilized proteins were separated in the first dimension in IPG (immobilized pH gradient) strips depending on their pI and subsequently in the second dimension according to their molecular weight by SDS-polyacrylamide gel electrophoresis. Of the more than 1500 protein spots visualized, several landmark spots were isolated from the gels and identified by either tryptic cleavage and subsequent MALDI-TOF mass spectrometry or by protein sequencing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 110, Issue 3, July 2005, Pages 191-195
Journal: Experimental Parasitology - Volume 110, Issue 3, July 2005, Pages 191-195
نویسندگان
David Leitsch, Christian Radauer, Katharina Paschinger, Iain B.H. Wilson, Heimo Breiteneder, Otto Scheiner, Michael Duchêne,