Growth dynamics of major microbial populations during biodegradation of o-phthalate in anaerobic sediment slurries under a CO2/H2 atmosphere

o-Phthalate transformers increased about five orders of magnitude (to 1.6 × 1011 cells g−1 sediment) just before the onset of fast biotransformation of o-phthalate (21.6 mg l−1) and then decreased sharply when the concentration of o-phthalate became low during biodegradation of o-phthalate in anaerobic sediment slurries under CO2/H2 (4:1, v/v). In contrast, the benzoate transformers increased about four orders of magnitude (to 1.6 × 1011 cells g−1 sediment) in 48 days and then increased one more order (to 1.6 × 1012 cells g−1 sediment) in 60 days and then remained at that high level in those sediment slurries. When making a comparison between the growth dynamics of o-phthalate transformers, acetogens, sulfate reducers, and methanogens and the time course of o-phthalate transformation, it appears that acetogens did not initiate biotransformation of o-phthalate, and that sulfate reducers and methanogens were not directly involved in o-phthalte degradation. o-Phthalate was not transformed in sediment slurries amended with BESA plus molybdate under CO2/H2.