Interfacial physicochemical properties of functionalized conducting polypyrrole particles

Reactivity of the PS-PPyNSE75 particles has been investigated using 2-aminoethanol and 2-mercaptoethanol, two model molecules bearing functional groups borne by proteins. Incubation of the particles with these model molecules clearly showed that the particles are highly reactive towards amine and thiol groups. The functionalized particles were then tested as bioadsorbents. PS-PPyNSE75 particles were found to be effective in attaching an aminated biotin. The Biotin-decorated PS-PPyNSE75 latex particles were incubated with avidin with a result of a significant change in the surface composition that is in line with the attachment of the protein by specific binding to biotin.