کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9573345 | 1388898 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Thermal denaturation of the BRCT tandem repeat region of human tumour suppressor gene product BRCA1
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Thermal denaturation of the BRCT tandem repeat region of human tumour suppressor gene product BRCA1 Thermal denaturation of the BRCT tandem repeat region of human tumour suppressor gene product BRCA1](/preview/png/9573345.png)
چکیده انگلیسی
Reduced stability of the tandem BRCT domains of human BReast CAncer 1 (BRCA1) due to missense mutations may be critical for loss of function in DNA repair and damage-induced checkpoint control. In the present thermal denaturation study of the BRCA1 BRCT region, high-precision differential scanning calorimetry (DSC) and circular dichroism (CD) spectroscopy provide evidence for the existence of a denatured state that is structurally very similar to the native. Consistency between theoretical structure-based estimates of the enthalpy (ÎH) and heat capacity change (ÎCp) and the calorimetric results is obtained when considering partial thermal unfolding contained in the region of the conserved hydrophobic pocket formed at the interface of the two BRCT repeats. The structural integrity of this region has been shown to be crucial for the interaction of BRCA1 with phosphorylated peptides. In addition, cancer-causing missense mutations located at the inter-BRCT-repeat interface have been linked to the destabilization of the tandem BRCT structure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 114, Issue 1, 1 April 2005, Pages 1-12
Journal: Biophysical Chemistry - Volume 114, Issue 1, 1 April 2005, Pages 1-12
نویسندگان
Serapion Pyrpassopoulos, Angela Ladopoulou, Metaxia Vlassi, Yannis Papanikolau, Constantinos E. Vorgias, Drakoulis Yannoukakos, George Nounesis,