کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9573620 | 1388908 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of nonenzymatic glycation on functional and structural properties of hemoglobin
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effect of nonenzymatic glycation on functional and structural properties of hemoglobin Effect of nonenzymatic glycation on functional and structural properties of hemoglobin](/preview/png/9573620.png)
چکیده انگلیسی
HbA1c, the major glycated hemoglobin increases proportionately with blood glucose concentration in diabetes mellitus. H2O2 promotes more iron release from HbA1c than that from nonglycated hemoglobin, HbA0. This free iron, acting as a Fenton reagent, might produce free radicals and degrade cell constituents. Here we demonstrate that in the presence of H2O2, HbA1c degrades DNA and protein more efficiently than HbA0. Formation of carbonyl content, an index of oxidative stress, is higher by HbA1c. Compared to HbA0, HbA1c is more rapidly autooxidized. Besides these functional changes, glycation also causes structural modifications of hemoglobin. This is demonstrated by reduced α-helix content, more surface accessible hydrophobic tryptophan residues, increased thermolability and weaker heme-globin linkage in HbA1c than in its nonglycated analog. The glycation-induced structural modification of hemoglobin may be associated with its functional modification leading to oxidative stress in diabetic patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 113, Issue 3, 1 March 2005, Pages 289-298
Journal: Biophysical Chemistry - Volume 113, Issue 3, 1 March 2005, Pages 289-298
نویسندگان
Subhrojit Sen, Manoj Kar, Anjana Roy, Abhay Sankar Chakraborti,