کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9605560 | 44166 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fluorometric detection of nitric oxide using 2,3-diaminonaphthalene incorporated in β-cyclodextrin
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aromatic diamino compound 2,3-diaminonaphthalene (DAN) has been used in numerous occasion to detect nitrite and nitric oxide in biological fluids; however, the sensitivity of this method relies on a strong alkalinisation of the sample which, in many cases, limits the general applicability of the method. In this work, we propose a new fluorescent method to quantify nitric oxide at physiological pH, based on the reaction of this radical with DAN, previously incorporated in β-cyclodextrin (β-CD). Changes in the fluorescence intensity of DAN were used to characterize the DAN/β-CD inclusion complexes and determine the association constant and stoichiometry of the process. Reactivity of the DAN/β-CD complex was then evaluated in presence of different concentrations of NO. Results show that the inclusion of DAN into the β-cyclodextrin does not prevent the formation of the fluorescent product 2,3-naphthotriazole (NATH), which remains into the cyclodextrin increasing considerably its fluorescence quantum yield, even at neutral pH. A plot between NO concentration and fluorescence intensity of NATH was built to determine the sensitivity of the DAN/β-CD complex to the radical. A linear relationship was found with a lower detection limit â¼20 nM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 173, Issue 3, 15 July 2005, Pages 384-389
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 173, Issue 3, 15 July 2005, Pages 384-389
نویسندگان
RocÃo Esquembre, Isabel Pastor, Ricardo Mallavia, C. Reyes Mateo,