کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9607657 | 44869 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Metabolic flux analysis of HEK-293 cells in perfusion cultures for the production of adenoviral vectors
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Metabolic flux analysis of HEK-293 cells in perfusion cultures for the production of adenoviral vectors Metabolic flux analysis of HEK-293 cells in perfusion cultures for the production of adenoviral vectors](/preview/png/9607657.png)
چکیده انگلیسی
To meet increasing needs of adenovirus vectors for gene therapy programs, development of efficient and reproducible production processes is required. Perfusion cultures were employed to allow infection at greater cell concentrations. In an effort to define culture conditions resulting in enhanced productivities, experiments performed at different feed rates and infected at various cell densities were compared using metabolic flux analysis. The highest specific product yields were achieved in experiments performed at high perfusion rates and/or low cell concentrations. The intracellular flux analysis revealed that these experiments exhibited greater glycolytic fluxes, slightly higher TCA fluxes, and greater ATP production rates at the time of infection. In contrast, cultures infected at high cell density and/or low medium renewal rates were characterized by a more efficient utilization of glucose at the time of infection, but the specific product yields achieved were lower. The intracellular flux analysis provided a rational basis for the implementation of a feeding strategy that allowed successful infection at a density of 5Ã106Â cells/ml.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 7, Issues 5â6, SeptemberâNovember 2005, Pages 467-476
Journal: Metabolic Engineering - Volume 7, Issues 5â6, SeptemberâNovember 2005, Pages 467-476
نویسندگان
O. Henry, M. Perrier, A. Kamen,