Yield and composition of different fractions obtained after enzymatic hydrolysis of cod (Gadus morhua) by-products

Use of Neutrase produced more dry FPH (23-57%) than Flavourzyme. The amount of oil fraction obtained after Neutrase treatment was up to 10% higher compared to Flavourzyme due to 13-60% smaller emulsion layer and higher degree of hydrolysis in sludge liberating more lipids from sludge to oil fraction. Emulsion, which is not a desirable fraction after hydrolysis can also be reduced or avoided by reduction or/and elimination of addition of water into hydrolysis mixture.