کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9617738 | 49168 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immobilization of cytochrome P-450 on MCM-41 with different silicon/aluminum ratios
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
کاتالیزور
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![عکس صفحه اول مقاله: Immobilization of cytochrome P-450 on MCM-41 with different silicon/aluminum ratios Immobilization of cytochrome P-450 on MCM-41 with different silicon/aluminum ratios](/preview/png/9617738.png)
چکیده انگلیسی
The catalytic activity of two cytochrome P-450 (CYP450) isoforms (CYP2C9 and CYP2B4, 1.14.14.1) immobilized on a mesoporous material “MCM-41” synthesized with Si/Al ratios of â, 80 and 100 was evaluated. The amount of CYP2C9 and CYP2B4 immobilized in each material was different, due to isoform size, the number of hydrogen bonds and the electrostatic interactions between the aminoacid residues and the Lewis acid sites of the material. The catalytic activity of immobilized CYP2C9 and CYP2B4 took place without using cytochrome P-450 reductase; consequently MCM-41 participates in electron transfer through the Lewis acid sites. In addition, the catalytic activity of immobilized CYP2B4 on MCM-41 was similar to its free form when a Si/Al ratio of 100 was used. Finally, although the catalytic activity of immobilized CYP2C9 was also better on MCM-41 with a Si/Al ratio of 100, it maintained around 30% of the catalytic activity in relation to its free form.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microporous and Mesoporous Materials - Volume 80, Issues 1â3, 2 May 2005, Pages 25-31
Journal: Microporous and Mesoporous Materials - Volume 80, Issues 1â3, 2 May 2005, Pages 25-31
نویسندگان
M.C. Rosales Hernández, J.E. Mendieta Wejebe, J.I. Vázquez Alcántara, R. Miranda Ruvalcaba, L.A. GarcıÌa Serrano, J. Trujillo Ferrara,