کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9617770 | 49168 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adsorption of Bovine Serum Albumin and lysozyme on siliceous MCM-41
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
کاتالیزور
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Adsorption of Bovine Serum Albumin and lysozyme on siliceous MCM-41 Adsorption of Bovine Serum Albumin and lysozyme on siliceous MCM-41](/preview/png/9617770.png)
چکیده انگلیسی
The adsorption of Bovine Serum Albumin (BSA) and lysozyme on MCM-41 was studied. In particular, characteristics important to the use of this adsorbent as a chromatographic support for protein separations have been examined. These include the effects of external surface area, solution conditions (pH), and rate of adsorption. Comparative studies were performed on MCM-41 of three pore sizes (24.8Â Ã
, 36Â Ã
, 53.5Â Ã
). It has been shown that differences in external surface area can strongly influence the adsorption capacity. This implies that adsorption on the external surface can be misinterpreted as adsorption in the pores of MCM-41. Thus, high adsorption capacities may not necessarily mean high molecular-sieving capacities. This paper also reports a dependence of protein uptake rates on solution conditions. Calorimetry suggests that under certain solution conditions the adsorption of BSA is a combination of two sequential energetic events. These are postulated to be BSA adsorption followed by reorientation/reconformation of the adsorbed protein on the surface. The slow second step affects the attainment of equilibrium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microporous and Mesoporous Materials - Volume 80, Issues 1â3, 2 May 2005, Pages 311-320
Journal: Microporous and Mesoporous Materials - Volume 80, Issues 1â3, 2 May 2005, Pages 311-320
نویسندگان
Amit Katiyar, Lei Ji, Panagiotis G. Smirniotis, Neville G. Pinto,