کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9743343 | 1491187 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A one-step method to construct a third-generation biosensor based on horseradish peroxidase and gold nanoparticles embedded in silica sol-gel network on gold modified electrode
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A one-step method for fabrication of horseradish peroxidase (HRP) biosensor has been developed. The gold nanoparticles and HRP were simultaneously embedded in silica sol-gel network on gold electrode surface in the presence of cysteine. The immobilized HRP exhibited direct electrochemical behavior toward the reduction of hydrogen peroxide. The heterogeneous electron transfer rate constant was evaluated to be 7.8 sâ1. The biosensor displayed an excellent elctrocatalytic response to the reduction of H2O2 without any mediator. The calibration range of H2O2 was from 1.6 μmol Lâ1 to 3.2 mmol Lâ1 and a detection limit of 0.5 μmol Lâ1 at a signal-to-noise ratio of 3. The KMapp value of HRP immobilized on the electrode surface was found to be 1.1 mmol Lâ1. The biosensor exhibited high sensitivity, rapid response and long-term stability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 553, Issues 1â2, 30 November 2005, Pages 196-200
Journal: Analytica Chimica Acta - Volume 553, Issues 1â2, 30 November 2005, Pages 196-200
نویسندگان
Junwei Di, Chunping Shen, Shaohua Peng, Yifeng Tu, Shujin Li,