Detection of boldenone and its major metabolites by liquid chromatography-tandem mass spectrometry in urine samples

After solid phase extraction of urine samples, detection is carried out by high performance liquid chromatography-tandem mass spectrometry in multiple reaction monitoring. The average recovery for all the investigated compounds is above 70%. The developed method is very easy, quick and highly specific. Linearity, precision, decision limit and detection capability were also evaluated.