کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9743389 | 1491189 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glucose oxidase-β-galactosidase hybrid biosensor based on glassy carbon electrode modified with mercury for lactose determination
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
An amperometric biosensor prepared by immobilization of β-galactosidase and glucose oxidase (GOD) onto a glassy carbon electrode coated with mercury thin film for lactose has been developed. The enzymes were immobilized by gelatin and glutaraldehyde onto mercury thin film electrode easily. The chronoamperometric detection of lactose was carried out at â0.2 V (versus Ag/AgCl) in 0.05 M citrate buffer (pH 5.5). Optimum pH and temperature, and thermal stability were investigated. Interference effects of some sugars and repeatability of the biosensor were also studied. The response to lactose was found to be linear range between 1 Ã 10â4 and 3.5 Ã 10â3 M. The biosensor was applied to the determination of lactose in milk samples and the results obtained with the biosensor were validated by Somogyi-Nelson method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 551, Issues 1â2, 17 October 2005, Pages 51-56
Journal: Analytica Chimica Acta - Volume 551, Issues 1â2, 17 October 2005, Pages 51-56
نویسندگان
Tüge GöktuÄ, Mustafa Kemal Sezgintürk, Erhan Dinçkaya,