کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9743407 1491189 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A rapid method for determination and confirmation of the thyreostats in milk and urine by matrix solid-phase dispersion and gas chromatography-mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A rapid method for determination and confirmation of the thyreostats in milk and urine by matrix solid-phase dispersion and gas chromatography-mass spectrometry
چکیده انگلیسی
A rapid and simple method for determination and confirmation of the thyreostats in milk and urine has been developed. The samples were extracted and purified by matrix solid-phase dispersion (MSPD) with silica gel as the solid support, and the thyreostats including tapazole, 2-thiouracil, 6-methyl-2-thiouracil, 6-propyl-2-thiouracil and 6-phenyl-2-thiouracil have been separated and detected by gas chromatography-mass spectrometry in selected ion mode with dimethylthiouracil as internal standard after derivatization with pentafluorobenzylbromide and N-methyl-N-(trimethylsilyl)-trifluoroacetamide. The derivatization reaction was conducted in a strong basic condition, which can improve the derivatization yields of the thyreostats in the presence of the matrix. The relative abundances of the selected ions in the chromatograms of the derivatives were stable and consistent, and can be used for the confirmatory purpose. The average recoveries of the five thyreostatic compounds in milk and urine were above 70% in most cases with the relative standard deviations between 2.1 and 7.9%. The limit of detection was 0.0016 μg g−1 for the thyreostats except for that of tapazole, which was 0.004 μg g−1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 551, Issues 1–2, 17 October 2005, Pages 184-191
نویسندگان
, , , , ,