کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9743444 | 1491190 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A simple and sensitive assay of nucleic acids based on the enhanced resonance light scattering of zwitterionics
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A simple and sensitive assay of nucleic acids based on the enhanced resonance light scattering of zwitterionics A simple and sensitive assay of nucleic acids based on the enhanced resonance light scattering of zwitterionics](/preview/png/9743444.png)
چکیده انگلیسی
A new assay of nucleic acids at nanogram level was established based on the enhanced resonance light scattering (RLS) signals of two zwitterionics cocamidopropyl hydroxysultaine (HSB) and lauryl betaine (BS-12). Under optimum conditions, the weak RLS signal of HSB is enhanced by nucleic acids, and the enhanced RLS intensity is proportional to the concentration of nucleic acids in the range of 0.02-7.3 mg lâ1 for calf thymus DNA and 0.01-8.6 mg lâ1 for fish sperm DNA. The detection limits were 1.5 ng mlâ1 for calf thymus DNA and 1.9 ng mlâ1 for fish sperm DNA. Plasmid DNA extracted from K-12-HB101 colt was determined with satisfactory results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 550, Issues 1â2, 26 September 2005, Pages 204-209
Journal: Analytica Chimica Acta - Volume 550, Issues 1â2, 26 September 2005, Pages 204-209
نویسندگان
Zhanguang Chen, Weifeng Ding, Fenglian Ren, Jinbin Liu, Yizeng Liang,