کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9743947 | 1491203 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Multi sulfonamide screening in porcine muscle using a surface plasmon resonance biosensor
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A binding protein displaying broad-spectrum cross-reactivity within the sulfonamide group was used in conjunction with a sulfonamide specific sensor chip and a surface plasmon resonance biosensor to develop a rapid broad spectrum screening assay for sulfonamides in porcine muscle. Results for 40 samples were available in just over 5 h after the completion of a simple sample preparation protocol. Twenty sulfonamide compounds were detected. Acetylated metabolites were not recognised by the binding protein. Limit of detection (mean-three times standard deviation value when n = 20) was calculated to be 16.9 ng gâ1 in tissue samples. Intra-assay precision (n = 10) was calculated at 4.3 %CV for a sample spiked at 50 ng gâ1 with sulfamethazine, 3.6 %CV for a sample spiked at 100 ng gâ1 with sulfamethazine, 7.2 %CV for a sample spiked at 50 ng gâ1 with sulfadiazine and 3.1 %CV for a sample spiked at 100 ng gâ1 with sulfadiazine. Inter-assay precision (n = 3) was calculated at 9.7 %CV for a sample spiked at 50 ng gâ1 with sulfamethazine, 3.8 %CV for a sample spiked at 100 ng gâ1 with sulfamethazine, 3.5 %CV for a sample spiked at 50 ng gâ1 with sulfadiazine and 2.8 %CV for a sample spiked at 100 ng gâ1 with sulfadiazine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 529, Issues 1â2, 24 January 2005, Pages 123-127
Journal: Analytica Chimica Acta - Volume 529, Issues 1â2, 24 January 2005, Pages 123-127
نویسندگان
Terry McGrath, Andrew Baxter, Julie Ferguson, Simon Haughey, Peter Bjurling,