کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9743952 1491203 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of avermectin and moxidectin residues in milk by liquid chromatography-tandem mass spectrometry using an atmospheric pressure chemical ionization/atmospheric pressure photoionization source
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Analysis of avermectin and moxidectin residues in milk by liquid chromatography-tandem mass spectrometry using an atmospheric pressure chemical ionization/atmospheric pressure photoionization source
چکیده انگلیسی
The avermectins-ivermectin (IVR), doramectin (DOR), eprinomectin (EPR)-and also the milbemycin moxidectin (MOX) are anthelmintic compounds that may be administered to cattle. Different ionization techniques, including atmospheric pressure photoionization (APPI), were evaluated for the detection of these residues in milk. The ionization response of these compounds using APPI was compared with that obtained by atmospheric pressure chemical ionization (APCI), a combination of APPI and APCI, and electrospray. It was found that the relative response of these drugs with different ionization protocols varied depending on the compound and the mobile phase. When monitoring negative ions, the use of the UV lamp increased the MS response. However, the best response for these compounds was obtained by operating the APCI/APPI source in the positive ion mode without any discharge current applied to the corona needle, whether the UV lamp was on or not. Using this mode of ionization, an MS-MS method was established to monitor the product ion scans of the sodiated molecular ions with an ion trap instrument. Milk fortified with these compounds (0.5-20 ng g−1) and milk samples from dosed animals were analyzed after isolating the residues with a simple solid phase extraction method. EPR, DOR and IVR were confirmed in all of the extracts analyzed. MOX was confirmed in all samples fortified at 5 ng g−1 or higher. Acceptable recoveries (≥60%) and relative standard deviations (R.S.D. values ≤ 20%) were observed for the residues at the following levels: EPR and IVR (1-20 ng g−1); DOR and MOX (5-20 ng g−1).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 529, Issues 1–2, 24 January 2005, Pages 159-165
نویسندگان
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