کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9743953 | 1491203 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Liquid chromatography-electrospray ionisation-mass spectrometry based method for the determination of estradiol benzoate in hair of cattle
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A liquid chromatography (LC)-based method with mass spectrometric (MS/MS) detection was developed for the determination of estradiol benzoate residues in hair of cattle. First hair samples were pulverized with a ball mill followed by treatment with the reducing agent Tris(2-carboxyethyl)phosphine hydrochloride (TCEP). After liquid/liquid extraction samples were further purified by solid-phase extraction. Finally samples were analyzed with LC-MS/MS using deuterated estradiol benzoate as internal standard. The method was validated following the latest EU guidelines for screening methods using blank hair samples spiked at 5 ng gâ1. The detection capability (CCβ) was less than 5 ng gâ1 and the decision limit (CCα) was 1.6 ng gâ1. The recovery was 27% at the 5 ng gâ1 level and the accuracy was 95%. Confirmation, with two MS/MS transitions, was possible at a concentration of 5 ng gâ1 or higher. Incurred samples obtained from different animal experiments were analyzed and the presence of estradiol benzoate was confirmed. Finally hair samples from different slaughterhouses were analyzed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 529, Issues 1â2, 24 January 2005, Pages 167-172
Journal: Analytica Chimica Acta - Volume 529, Issues 1â2, 24 January 2005, Pages 167-172
نویسندگان
H. Hooijerink, A. Lommen, P.P.J. Mulder, J.A. van Rhijn, M.W.F. Nielen,