کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9745079 | 1645297 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The bacterial HPr kinase/phosphorylase: A new type of Ser/Thr kinase as antimicrobial target
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کلمات کلیدی
PEPCCPAHPRHprK/PHPr kinase/phosphorylasecatabolite control protein ACrystal - بلورP-loop - حلقه Pphosphate binding loop - حلقه اتصال فسفاتStructure - ساختار یا سازهactive site - سایت فعالinorganic phosphate - فسفات معدنیphosphoenolpyruvate - فسفوآنولپیرواتMechanism - مکانیسم یا سازوکارComplex - کمپلکس
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The bacterial HPr kinase/phosphorylase: A new type of Ser/Thr kinase as antimicrobial target The bacterial HPr kinase/phosphorylase: A new type of Ser/Thr kinase as antimicrobial target](/preview/png/9745079.png)
چکیده انگلیسی
Protein phosphorylation plays a major role in bacterial cellular regulation as in eukaryotes. The HPr Kinase/Phosphorylase (HprK/P) was the first bacterial serine protein kinase to have had its structure determined, establishing that it is unrelated to the eukaryotic kinases. HprK/P belongs to another large structural family, the P-loop containing proteins. Among them, P-loop containing kinases have been assumed to only phosphorylate small molecules, but the example of HprK/P suggests that some may have proteins as substrates, defining novel cellular signal transduction pathways. Another major result of the studies presented here is that HprK/P also catalyses the phosphorolysis of the phosphoserine, yielding serine and pyrophosphate. The two different catalytic activities are carried out at the same active site. The determination of the structure of the complex with the protein substrates HPr and PserHPr allowed us to propose a catalytic mechanism. Since regulation of HPr phosphorylation has been shown to be involved in the virulence process of pathogenic bacteria, a search for specific inhibitors of HprK/P is of clinical interest and the first hit has already been found.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1754, Issues 1â2, 30 December 2005, Pages 126-131
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1754, Issues 1â2, 30 December 2005, Pages 126-131
نویسندگان
Sylvie Nessler,