کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9748634 | 1493787 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of stable carbon isotope analysis to the detection of 17β-estradiol administration to cattle
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The use of anabolic agents in food producing animals is prohibited within the EU since 1988 (96/22/EC directive). The control of the illegal use of natural steroid hormones in cattle is still an exciting analytical challenge as far as no definitive method and non-ambiguous analytical criteria are available. The ability of gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) to demonstrate the administration of 17β-estradiol to bovine has been investigated in this paper. By comparison of 13C/12C isotopic ratio of main urinary estradiol metabolite, i.e. 17α-estradiol, with two endogenous reference compounds (ERCs), i.e. dehydroepiandrosterone (DHEA) and 5-androstene-3β,17α-diol, the differentiation of estradiol metabolite origin, either endogenous or exogenous, has been proved to be achievable. After treatment, the δ13CVPDB-values of 17α-estradiol reached â27â° to â29â°, whereas δ13CVPDB-values of DHEA remained between â13â° and â20â° depending on the diet, maize and grass, respectively. A significant difference of δ13CVPDB between ERCs and 17α-estradiol was measurable over a period of 2 weeks after estradiol ester administration to the animal.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1093, Issues 1â2, 4 November 2005, Pages 69-80
Journal: Journal of Chromatography A - Volume 1093, Issues 1â2, 4 November 2005, Pages 69-80
نویسندگان
C. Buisson, M. Hebestreit, A. Preiss Weigert, K. Heinrich, H. Fry, U. Flenker, S. Banneke, S. Prevost, F. Andre, W. Schaenzer, E. Houghton, B. Le Bizec,