کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9748761 | 1493791 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-performance liquid chromatography with electrochemical detection for analysis of gliclazide in plasma
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A sensitive HPLC-electrochemical detection method was developed for the analysis of gliclazide (GL) in human plasma. After deproteination of 100 μL of plasma by acetonitrile, evaporation, and reconstitution, GL was separated on a C18 column (150 mm Ã 4.6 mm) by the mobile phase (70 mM disodium tetraborate, pH 7.5, containing 26.5% of acetonitrile). The regression equations were linear (r > 0.9990) over the range of 50 nM to 4.00 μM. The precision and accuracy of intra- and inter-day analysis were less than 5.3 and 0.93% for relative standard deviation and relative error, respectively. The limit of detection for plasma was 10 nM for GL (S/N = 3, 10 μL injection). This newly developed method was applied for monitoring blood levels with one healthy volunteer dosing with a GL tablet.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1088, Issues 1â2, 23 September 2005, Pages 131-135
Journal: Journal of Chromatography A - Volume 1088, Issues 1â2, 23 September 2005, Pages 131-135
نویسندگان
Chien-Yuan Kuo, Shou-Mei Wu,