کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9748825 | 1493793 | 2005 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comprehensive two-dimensional gas chromatography with isotope dilution time-of-flight mass spectrometry for the measurement of dioxins and polychlorinated biphenyls in foodstuffs
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کلمات کلیدی
High-resolution mass spectrometry (HRMS)Comprehensive two-dimensional gas chromatography (GC × GC)Polychlorinated dibenzofurans (PCDFs) - دی انزو فورانهای پلی کلر (PCDFs)Polychlorinated dibenzo-p-dioxins (PCDDs) - دیابنزوپو دی اکسین های پلی کلر (PCDDs)Polychlorinated biphenyls (PCBs) - دیفنیلهای پلی کلر (PCBs)Time-of-flight mass spectrometry (TOF-MS) - طیف سنجی جرمی زمان (TOF-MS)
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC Ã GC-TOF-MS) experimental setup was tested for the measurement of seven 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins (PCDDs), ten 2,3,7,8-substituted polychlorinated dibenzofurans (PCDFs), four non-ortho-polychlorinated biphenyls (PCBs), eight mono-ortho-PCBs, and six indicator PCBs (Aroclor 1260) in foodstuff samples. A 40 m RTX-500 (0.18 mm I.D., 0.10 μm df) was used as the first dimension (1D) and a 1.5 m BPX-50 (0.10 mm I.D., 0.10 μm df) as the second dimension (2D). The GC Ã GC chromatographic separation was completed in 45 min. Quantification was performed using 13C-label isotope dilution (ID). Isotope ratios of the selected quantification ions were checked against theoretical values prior to peak assignment and quantification. The dynamic working range spanned three orders of magnitude. The lowest detectable amount of 2,3,7,8-TCDD was 0.2 pg. Fish, pork, and milk samples were considered. On a congener basis, the GC Ã GC-ID-TOF-MS method was compared to the reference GC-ID high resolution mass spectrometry (HRMS) method and to the alternative GC-ID tandem-in-time quadrupole ion storage mass spectrometry (QIST-MS/MS). PCB levels ranged from low picogram (pg) to low nanogram (ng) per gram of sample and data compared very well between the different methods. For all matrices, PCDD/Fs were at a low pg level (0.05-3 pg) on a fresh weight basis. Although congener profiles were accurately described, RSDs of GC Ã GC-ID-TOF-MS and GC-QIST-MS/MS were much higher than for GC-ID-HRMS, especially for low level pork and milk. On a toxic equivalent (TEQ) basis, all methods, including the dioxin-responsive chemically activated luciferase gene expression (DR-CALUX) assay, produced similar responses. A cost comparison is also presented.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1086, Issues 1â2, 9 September 2005, Pages 45-60
Journal: Journal of Chromatography A - Volume 1086, Issues 1â2, 9 September 2005, Pages 45-60
نویسندگان
Jean-François Focant, Gauthier Eppe, Marie-Louise Scippo, Anne-Cécile Massart, Catherine Pirard, Guy Maghuin-Rogister, Edwin De Pauw,