کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9748898 | 1493795 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mass spectrometric characterization of human skin elastin peptides produced by proteolytic digestion with pepsin and thermitase
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Mass spectrometric characterization of human skin elastin peptides produced by proteolytic digestion with pepsin and thermitase Mass spectrometric characterization of human skin elastin peptides produced by proteolytic digestion with pepsin and thermitase](/preview/png/9748898.png)
چکیده انگلیسی
This study investigated peptides resulting from the digestion of human skin elastin with pepsin and thermitase. Characterization of the peptides was performed using two complementary mass spectrometric techniques; LC/ESI-ion trap and nano-ESI-qTOF MS. 155 different peptides were identified using a combined database based and de novo sequencing approach resulting in a total sequence coverage of 65.4% calculated on the basis of the precursor tropoelastin (accession number A32707). A potential hydroxylation was found in 29% of the recovered prolines. Furthermore, the absence of amino acids expressed by exon 26A could be confirmed. However, contrary to earlier studies, amino acids expressed by exon 22 seem to exist.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1083, Issues 1â2, 12 August 2005, Pages 120-126
Journal: Journal of Chromatography A - Volume 1083, Issues 1â2, 12 August 2005, Pages 120-126
نویسندگان
Christian E.H. Schmelzer, Melkamu Getie, Reinhard H.H. Neubert,