کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9748963 1493797 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dynamic pH junction technique for on-line preconcentration of peptides in capillary electrophoresis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Dynamic pH junction technique for on-line preconcentration of peptides in capillary electrophoresis
چکیده انگلیسی
A method based on the presence of a dynamic pH junction within the capillary to induce band narrowing for enhanced detection sensitivity for some peptides is presented. This technique is predicated on a sharp reduction in an analyte's migration velocity following a reversal of its electrophoretic direction from the acidic sample zone to the basic BGS zone. Larger-than-usual injection volumes of samples in relatively high-conductivity matrices were enabled, without degrading peak shape, resolution and efficiency. The size of the original sample plug was reduced by as much as 38-fold, and improvement in detector response in terms of peak height by as much as 124-fold was obtained. The effects of pH and concentration of the sample matrix, and the length of sample injection on the efficiency of the technique are discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1079, Issues 1–2, 24 June 2005, Pages 266-273
نویسندگان
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