کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9749216 | 1493804 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quality evaluation of Flos Lonicerae through a simultaneous determination of seven saponins by HPLC with ELSD
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A new HPLC coupled with evaporative light scattering detection (ELSD) method has been developed for the simultaneous quantitative determination of seven major saponins, namely macranthoidin B (1), macranthoidin A (2), dipsacoside B (3), hederagenin-28-O-β-d-glucopyranosyl(6â1)-O-β-d-glucopyranosyl ester (4), macranthoside B (5), macranthoside A (6), and hederagenin-3-O-α-l-arabinopyranosyl(2â1)-O-α-l-rhamnopyranoside (7) in Flos Lonicerae, a commonly used traditional Chinese medicine (TCM) herb. Simultaneous separation of these seven saponins was achieved on a C18 analytical column. The mobile phase consisted of (A) acetonitrile-acetic acid (95:0.5) and (B) 0.5% aqueous acetic acid using a gradient elution of 29%A at 0-10 min, 29-46%A at 10-25 min and 46%A at 25-30 min. The drift tube temperature of ELSD was set at 106 °C, and with the nitrogen flow-rate of 2.6 l/min. All calibration curves showed good linear regression (r2 > 0.9922) within test ranges. This method showed good reproducibility for the quantification of these seven saponins in Flos Lonicerae with intra- and inter-day variations of less than 3.0% and 6.0%, respectively. The validated method was successfully applied to quantify seven saponins in five sources of Flos Lonicerae, which provides a new basis of overall assessment on quality of Flos Lonicerae.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1070, Issues 1â2, 8 April 2005, Pages 43-48
Journal: Journal of Chromatography A - Volume 1070, Issues 1â2, 8 April 2005, Pages 43-48
نویسندگان
Xing-Yun Chai, Song-Lin Li, Ping Li,