کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9753147 | 1494195 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microdialysis sampling coupled to on-line high-performance liquid chromatography for determination of arbutin in whitening cosmetics
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
We have developed a method that uses on-line microdialysis sampling coupled with high-performance liquid chromatography (HPLC) to determine arbutin in whitening cosmetics. The optimum analytical conditions for microdialysis sampling were a probe length of 10 mm and a dialysis flow-rate of 5 μl minâ1. The accuracy (% bias) for intra-day (n = 6) and inter-day (n = 30, five consecutive days) analyses ranged from â8.9 to 11.5%, with a precision below 7.64% R.S.D. The calibration curve was linear within the range from 0.1 to 20 mM (R2 = 0.9989). The detection limit was 15 μM. By comparing the arbutin levels determined this way in the whitening cosmetics with the results obtained from the no-net-flux method, we conclude that our proposed on-line microdialysis-HPLC system displays good accuracy. We evaluated the robustness of our optimum conditions by means of a Plackett-Burman design. Apart from the effect of a low flow-rate of perfusate - an increase of 12.52 ± 2.31% - we observed no significant changes in the analyses upon changing the levels of any other parameter. Because this on-line method offers the advantages of simplicity, reliability, the lack of any tedious sample pretreatment process, and a reduced use of organic solvents, we believe that it is suitable for the routine analysis of commercial cosmetics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 829, Issues 1â2, 27 December 2005, Pages 149-152
Journal: Journal of Chromatography B - Volume 829, Issues 1â2, 27 December 2005, Pages 149-152
نویسندگان
Cheng-Hui Lin, Hsin-Lung Wu, Yeou-Lih Huang,