کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9753167 | 1494196 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of glabridin in human plasma by solid-phase extraction and LC-MS/MS
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of glabridin in human plasma by solid-phase extraction and LC-MS/MS Determination of glabridin in human plasma by solid-phase extraction and LC-MS/MS](/preview/png/9753167.png)
چکیده انگلیسی
Glabridin is a major flavonoid included specifically in licorice (Glycyrrhiza glabra L.), and has various physiological activities including antioxidant and anti-inflammatory effects. We have developed and validated an analytical method for determination of glabridin in human plasma by solid-phase extraction (SPE) and LC-MS/MS. Glabridin was extracted from plasma by SPE using a C8 cartridge and analyzed by LC-MS/MS using mefenamic acid as an internal standard (IS). The analyte were separated by a C18 column on LC, and monitored with a fragment ion of m/z 201 formed from a molecular ion of m/z 323 for glabridin and that of m/z 196 from m/z 240 for IS during negative ion mode with tandem MS detection. The lower limit of quantitation (LLOQ) of glabridin was 0.1 ng/mL in plasma, corresponding to 1.25 pg injected on-column. The calibration curves exhibited excellent linearity (r > 0.997) between 0.1 and 50 ng/mL. Precision and accuracy were <17 and <±7% at LLOQ, and <11 and <±5% at other concentrations. Glabridin was recovered >90%, and was stable when kept at 10 °C for 72 h, at â20 °C until 12 weeks, and after three freeze-thaw cycles. This is the first report on determination of glabridin in body fluids by the selective, sensitive, and reproducible method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 828, Issues 1â2, 15 December 2005, Pages 70-74
Journal: Journal of Chromatography B - Volume 828, Issues 1â2, 15 December 2005, Pages 70-74
نویسندگان
Fumiki Aoki, Kaku Nakagawa, Akiyoshi Tanaka, Kazue Matsuzaki, Naoki Arai, Tatsumasa Mae,