کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9753208 | 1494197 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of dihydroxynaphthalenes in human urine by gas chromatography-mass spectrometry
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کلمات کلیدی
CyPTMSDHNtrimethylsilylGSDPAHGC–MS - GC-MSUrine - ادرار geometric standard deviation - انحراف استاندارد هندسیBiomarker - بیومارکرElectron impact - تاثیر الکترونیDihydroxynaphthalene - دی هیدروکسین فتالینCytochrome P450 - سیتوکروم پی۴۵۰SIM - سیم کارتgas chromatography–mass spectrometry - طیف سنجی جرم کروماتوگرافی گازLod - لودlimit of detection - محدودیت تشخیصGeometric mean - میانگین هندسیselected ion monitoring - نظارت بر یون انتخاب شدهNaphthalene - نفتالینPolycyclic aromatic hydrocarbon - هیدروکربن آروماتیک چند حلقه ای
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of dihydroxynaphthalenes in human urine by gas chromatography-mass spectrometry Determination of dihydroxynaphthalenes in human urine by gas chromatography-mass spectrometry](/preview/png/9753208.png)
چکیده انگلیسی
A gas chromatography-mass spectrometry (GC-MS) method was developed for measuring 1,2-dihydroxynaphthalene (1,2-DHN) and 1,4-dihydroxynaphthalene (1,4-DHN) in urine. The method involves enzymatic digestion of urinary conjugates to release the DHNs which were then analyzed as trimethylsilyl derivatives by GC-MS. For 1,2-DHN and 1,4-DHN, respectively, the assay limits of detection were 0.21 and 0.15 μg/l, the assay limits of quantitation were 0.69 and 0.44 μg/l, and the coefficients of variation were 14.7 and 10.9%. This method was successfully applied to determine urinary levels of 1,2-DHN and 1,4-DHN in coke workers (14 top workers and 13 side-bottom workers) and 21 matching control workers from the steel industry of northern China. The geometric mean (GM) levels of 1,2-DHN were approximately 100 and 30 times higher than those of 1,4-DHN in exposed and control subjects, respectively. The GM levels 1,2-DHN and 1,4-DHN were significantly higher for coke workers (1,2-DHN: top workers - 552 μg/l, side-bottom workers - 260 μg/l; 1,4-DHN: top workers - 3.42 μg/l, side-bottom workers - 3.56 μg/l) than for controls (1,2-DHN: 38.8 μg/l; 1,4-DHN: 1.21 μg/l) (p â¤Â 0.0031). In each exposure category, levels of the DHNs were marginally greater in smokers than in nonsmokers (p = 0.0646). Strong correlations were observed among 1,2-DHN and 1,4-DHN and previously measured urinary levels of naphthalene, 1-hydroxynaphthalene, and 2-hydroxynaphthalene in these subjects (rs â¥Â 0.623; p < 0.0001). Also, levels of 1,2-DHN were significantly correlated with those of serum albumin adducts of l,2-naphthoquinone (rs = 0.492, p = 0.0004). These results indicate that 1,2- and 1,4-DHN are good biomarkers for assessment of naphthalene exposure in coke workers. Since the DHNs are precursors of the naphthoquinones, which have been implicated as toxic products of naphthalene metabolism, measurements of urinary DHNs may have toxicological significance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 826, Issues 1â2, 5 November 2005, Pages 206-213
Journal: Journal of Chromatography B - Volume 826, Issues 1â2, 5 November 2005, Pages 206-213
نویسندگان
Renan Wu, Suramya Waidyanatha, Alistair P. Henderson, Berrin Serdar, Yuxin Zheng, Stephen M. Rappaport,