کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9753288 | 1494199 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of telithromycin in human plasma and microdialysates by high-performance liquid chromatography
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A high-performance liquid chromatography method for the quantitative determination of telithromycin in biological fluids is described. The method is suitable for plasma and microdialysates from the interstitial space fluid of skeletal muscle and subcutaneous adipose tissue. Plasma samples were deproteinised with trichloroacetic acid and neutralised with sodium hydroxide. Microdialysates were analysed without further preparation step. Telithromycin was separated isocratically on a reverse-phase column using acetonitrile-0.03 M ammonium acetate, pH 5.2 (43:57, v/v) at a flow rate of 0.8 ml minâ1, and fluorescence detection (excitation 263 nm, emission 460 nm). The calibration curve was linear from 0.01 to 5 μg mlâ1. Within- and between-day imprecision and inaccuracy was â¤10%. The limits of quantification were 0.02 and 0.015 μg mlâ1 for plasma and microdialysates, respectively. Since telithromycin is decomposed in aqueous solution at ambient temperature, it is strongly recommended to store samples frozen at â80 °C, to maintain the temperature at 4 °C during all preparation steps, and to analyse samples within 120 min after thawing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 822, Issues 1â2, 5 August 2005, Pages 133-136
Journal: Journal of Chromatography B - Volume 822, Issues 1â2, 5 August 2005, Pages 133-136
نویسندگان
Friederike Traunmüller, Rainer Gattringer, Markus A. Zeitlinger, Wolfgang Graninger, Markus Müller, Christian Joukhadar,